CatWalk XT is a complete solution for the quantitative assessment of footfalls and gait in rodents, validated in research on spinal cord injury, neuropathic pain, arthritis, stroke, Parkinson’s, ataxia, traumatic brain injury, and peripheral nerve damage. Footprints are captured while a rat or mouse voluntarily traverses a glass plate towards a goal box. The turn-key system consists of a corridor that directs the free movement of the subject in a straight line, a hardened glass walkway that enables CatWalk XT’s Illuminated Footprints technology, and a high speed (100 fps) color camera for extremely accurate spatial and temporal resolution. With the Illuminated Footprints technology, the software identifies subtle changes within the dimensions, position and timing of each footprint. Over 100 parameters are calculated for qualitative and quantitative analysis of individual footfalls and gait.