The UID Rodent Matrix is an RFID reader board placed under each cage to record location, movement and temperature. The system enables noninvasive, undisturbed and continuous monitoring of group-housed rodents in a home cage setting. The data is automatically collected, and is available throughout the course of the study in real-time. The system provides a practical and flexible solution in a scalable framework to fit all needs and budgets. The system is compatible for monitoring as little as one static cage and scaling up for monitoring hundreds of cages from high-density IVC rack systems.